vectashield mounting medium (Vector Laboratories)
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Vectashield Mounting Medium, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 22145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vectashield+medium+with+dapi/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/pm42898335-91-74-77
Average 98 stars, based on 22145 article reviews
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other:Article Title: Description of the Seminiferous Epithelium Cycle Stages in the Zebra Finch Taeniopygia guttata . Article Snippet: Following post-hybridization washes, slides were mounted in Incubation:Article Title: TBXA2R activates ERMs to drive motility, invasion, and metastatic colonization of TNBC cells Article Snippet: Cells were then washed with PBS and fixed with 10% trichloroacetic acid (#T0699; Sigma-Aldrich) for 10 min at room temperature, followed by extensive washes with TBS (20 mM Tris–HCl, pH 7.5, 154 mM NaCl, 2 mM EGTA, 2 mM MgCl 2 ). .. Cells were then permeabilized with 0.02% saponin (#0163; Amresco) diluted in TBS supplemented with 2% BSA (#ALB001.250; BioShop) for 1 h. Cells were then incubated overnight with rabbit anti-p-ERM primary antibody (1:5,000; ) or anti-p-myosin II antibody (1:200, #3671; Cell Signaling Technology) followed by goat anti-rabbit Alexa Fluor 488–conjugated secondary antibody (1:200, #A11070; Invitrogen) for 1 h. Glass coverslips were finally mounted in Article Title: TDP-43 pathology is linked to motor neuron loss but is independent of stress granules in vivo Article Snippet: TDP-43 aptamer (1/100, TDP-43 Apt CGGUGUUGCU with a 3’ Biotin-TEG modification, ATDBio, Southampton, UK) prepared in protein block was applied to the tissue and incubated for 3 h at 4 °C followed by incubation with 4% PFA overnight at 4 °C. .. Sections were incubated with primary and secondary antibodies (1/100 goat-anti biotin Ab6652, 1/200 A647 donkey anti-goat Ab150135) then washed and mounted with Article Title: TBXA2R activates ERMs to drive motility, invasion, and metastatic colonization of TNBC cells. Article Snippet: Cells were then washed with PBS and fixed with 10% trichloroacetic acid (#T0699; Sigma-Aldrich) for 10 min at room temperature, followed by extensive washes with TBS (20 mM Tris–HCl, pH 7.5, 154 mM NaCl, 2 mM EGTA, 2 mM MgCl2). .. Cells were then permeabilized with 0.02% saponin (#0163; Amresco) diluted in TBS supplemented with 2% BSA (#ALB001.250; BioShop) for 1 h. Cells were then incubated overnight with rabbit anti-p-ERM primary antibody (1:5,000; Roubinet et al, 2011) or anti-p-myosin II antibody (1:200, #3671; Cell Signaling Technology) followed by goat anti-rabbit Alexa Fluor 488–conjugated secondary antibody (1:200, #A11070; Invitrogen) for 1 h. Glass coverslips were finally mounted in Microscopy:Article Title: TBXA2R activates ERMs to drive motility, invasion, and metastatic colonization of TNBC cells Article Snippet: Cells were then washed with PBS and fixed with 10% trichloroacetic acid (#T0699; Sigma-Aldrich) for 10 min at room temperature, followed by extensive washes with TBS (20 mM Tris–HCl, pH 7.5, 154 mM NaCl, 2 mM EGTA, 2 mM MgCl 2 ). .. Cells were then permeabilized with 0.02% saponin (#0163; Amresco) diluted in TBS supplemented with 2% BSA (#ALB001.250; BioShop) for 1 h. 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In teleost fish, Aromatase B (AroB), encoded by cyp19a1b, is brainspecific and highly expressed during early development, suggesting a role in neurogenesis.. Here, we investigated the role of AroB in zebrafish eye development using morpholino (MO)-mediated knockdown. Staining:Article Title: Alzheimer's disease target and drug discovery by leveraging multiomics and electronic health data. Article Snippet: The sections were treated with 0.5% Triton X-100 for 30 min and blocked in 10% fetal bovine serum (FBS) for 1 h. For organoid sections, anti-amyloid (B-4) Alexa Fluor 488 conjugated antibody (1:200; Santa Cruz Biotechnology; sc-28365) and anti-tau (phospho T205) Alexa Fluor 647 conjugated antibody (1:200; Abcam; AB307376) were used to detect amyloid-β and phosphorylated tau, respectively. .. Antibodies were stained for 2 h at room temperature and washed three times with phosphate-buffered saline (PBS) (20 min each) before mounting in Saline:Article Title: Alzheimer's disease target and drug discovery by leveraging multiomics and electronic health data. Article Snippet: The sections were treated with 0.5% Triton X-100 for 30 min and blocked in 10% fetal bovine serum (FBS) for 1 h. For organoid sections, anti-amyloid (B-4) Alexa Fluor 488 conjugated antibody (1:200; Santa Cruz Biotechnology; sc-28365) and anti-tau (phospho T205) Alexa Fluor 647 conjugated antibody (1:200; Abcam; AB307376) were used to detect amyloid-β and phosphorylated tau, respectively. .. Antibodies were stained for 2 h at room temperature and washed three times with phosphate-buffered saline (PBS) (20 min each) before mounting in |
